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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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renal cortex proximal tubule epithelial cells  (ATCC)
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Surface function in <t>Imaris</t> for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic <t>lesion;</t> aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.
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Image Search Results


Surface function in Imaris for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic lesion; aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.

Journal: STAR Protocols

Article Title: Protocol for mouse vascular dementia model and evaluation of progressive tissue damage in subcortical white matter and adjacent cortex

doi: 10.1016/j.xpro.2026.104396

Figure Lengend Snippet: Surface function in Imaris for quantification of core size, axon volume and its myelination (A) Create and name the surfaces for core, MBP_core and NF160_core. (B) Masking the core_MBP and core_NF160 channels. (C) A list of parameters for analyzing MBP or NF160 surfaces under “creation” icon. Make sure all the images are analyzed using the same series of parameters. (D) Representative images showing (left to right, top to bottom): Co-staining of NF160 (white), MBP (green), and DAPI (blue) in subcortical WM ischemic lesion; aggregation of nuclei in the lesion core shown by DAPI staining; Core surface; breakdown and debris of neurofilament shown by NF160 staining; debris of myelination damage shown by MBP staining; core axons classified by the extent of myelination.

Article Snippet: Quantification and classification of neuron subtypes in lesion adjacent cortex in Imaris (A) (Left) Masking Satb2 (green) channel in lesion adjacent cortex. (Middle) Aggregation of nucleus (DAPI+, blue) in ischemic lesion core in VaD mouse brain. (Right) Masking NeuN (white) channel in lesion adjacent cortex. (B) Creation of surfaces for core and ipsi_ctx (ipsi lateral cortex), spots for neun_ipsi, etc. (C) Combined core surface, masked NeuN+ channel in lesion adjacent cortex, and spot classification of NeuN+ cells by distance away from core. (D) Spot classification of NeuN+ cells by distance away from core.

Techniques: Staining

Quantification and classification of neuron subtypes in lesion adjacent cortex in Imaris (A) (Left) Masking Satb2 (green) channel in lesion adjacent cortex. (Middle) Aggregation of nucleus (DAPI+, blue) in ischemic lesion core in VaD mouse brain. (Right) Masking NeuN (white) channel in lesion adjacent cortex. (B) Creation of surfaces for core and ipsi_ctx (ipsi lateral cortex), spots for neun_ipsi, etc. (C) Combined core surface, masked NeuN+ channel in lesion adjacent cortex, and spot classification of NeuN+ cells by distance away from core. (D) Spot classification of NeuN+ cells by distance away from core. Scale bars = 100 μm.

Journal: STAR Protocols

Article Title: Protocol for mouse vascular dementia model and evaluation of progressive tissue damage in subcortical white matter and adjacent cortex

doi: 10.1016/j.xpro.2026.104396

Figure Lengend Snippet: Quantification and classification of neuron subtypes in lesion adjacent cortex in Imaris (A) (Left) Masking Satb2 (green) channel in lesion adjacent cortex. (Middle) Aggregation of nucleus (DAPI+, blue) in ischemic lesion core in VaD mouse brain. (Right) Masking NeuN (white) channel in lesion adjacent cortex. (B) Creation of surfaces for core and ipsi_ctx (ipsi lateral cortex), spots for neun_ipsi, etc. (C) Combined core surface, masked NeuN+ channel in lesion adjacent cortex, and spot classification of NeuN+ cells by distance away from core. (D) Spot classification of NeuN+ cells by distance away from core. Scale bars = 100 μm.

Article Snippet: Quantification and classification of neuron subtypes in lesion adjacent cortex in Imaris (A) (Left) Masking Satb2 (green) channel in lesion adjacent cortex. (Middle) Aggregation of nucleus (DAPI+, blue) in ischemic lesion core in VaD mouse brain. (Right) Masking NeuN (white) channel in lesion adjacent cortex. (B) Creation of surfaces for core and ipsi_ctx (ipsi lateral cortex), spots for neun_ipsi, etc. (C) Combined core surface, masked NeuN+ channel in lesion adjacent cortex, and spot classification of NeuN+ cells by distance away from core. (D) Spot classification of NeuN+ cells by distance away from core.

Techniques: